Plasmid_Backbone

Part:BBa_K1138002:Design

Designed by: Rachit Jain   Group: iGEM13_UGA-Georgia   (2013-09-06)


pAW50-linker-mcherry(+RBS) (ugaiGEM12)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 199
    Illegal EcoRI site found at 205
    Illegal XbaI site found at 220
    Illegal SpeI site found at 975
    Illegal PstI site found at 615
    Illegal PstI site found at 982
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 199
    Illegal EcoRI site found at 205
    Illegal SpeI site found at 975
    Illegal PstI site found at 615
    Illegal PstI site found at 982
    Illegal NotI site found at 211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 199
    Illegal EcoRI site found at 205
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 205
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 199
    Illegal SpeI site found at 975
    Illegal PstI site found at 615
    Illegal PstI site found at 982
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 205
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 199
    Illegal XbaI site found at 220
    Illegal SpeI site found at 975
    Illegal PstI site found at 615
    Illegal PstI site found at 982
    Illegal AgeI site found at 993
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3151
    Illegal BsaI.rc site found at 1534
    Illegal BsaI.rc site found at 1863
    Illegal SapI site found at 4815


Design Notes

Primers were designed in a way to introduce EcoRI-NotI-XbaI-RBS- NsiI sites before the linker.


Source

mcherry was obtained from Discosoma sp.

References